The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner.

F Brunel; M M Zakin; H Buc; M Buckle

doi:10.1093/nar/24.9.1608

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The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner.

F Brunel·M M Zakin·H Buc·M Buckle

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Abstract

Polypyrimidine tract binding (PTB) protein is a cellular factor whose function is unknown. Various RNA or single-stranded DNA sequences have been shown to interact with PTB. In this paper, using laser UV crosslinking and electrophoretic mobility shift assays to probe DNA-protein interactions, we demonstrate that PTB binding at a single-stranded DNA target is highly sequence-specific. We provide data showing that PTB interacts with the top strand of the adenovirus major late promoter transcriptional initiator, a sequence rich in pyrimidine residues. We also demonstrate that PTB is organised into at least two different binding domains.

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Adenoviridae Base Sequence Binding, Competitive Cross-Linking Reagents DNA, Single-Stranded DNA-Binding Proteins Escherichia coli Kinetics Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides Plasmids Polypyrimidine Tract-Binding Protein Promoter Regions, Genetic RNA-Binding Proteins Recombinant Fusion Proteins Ultraviolet Rays Journal Article Research Support, Non-U.S. Gov't

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F Brunel, M M Zakin, H Buc, M Buckle. 1996-05-01. The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner.. https://doi.org/10.1093/nar%2F24.9.1608

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