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LPS does not directly induce STAT activity in mouse macrophages.

W Deng·Y Ohmori·T A Hamilton
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Abstract

Induction of gene expression in cytokine-treated cells involves the protein tyrosine kinase-dependent activation of members of the STAT family of transcription factors. To determine if lipopolysaccharide (LPS) might activate one or more STAT factors, nuclear extracts from LPS-treated RAW264.7 macrophages were assayed for STAT-like DNA binding activity using oligonucleotides recognized by different members of this protein family. Within 30 min a single LPS-inducible DNA-protein complex was detected using three separate oligonucleotides. This activity was not reactive with anti-STAT antibodies and was subsequently identified as composed of the NF kappa B components NF kappa B1 and Rel-A. Thus, LPS does not directly stimulate STAT factors with known sequence-specific DNA binding activity.

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AnimalsAntibodiesBase SequenceCell LineDNA-Binding ProteinsLipopolysaccharidesMacrophagesMiceMolecular Sequence DataNF-kappa BProtein BindingSTAT1 Transcription FactorSignal TransductionTrans-ActivatorsJournal Article

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W Deng, Y Ohmori, T A Hamilton. 1996-05-25. LPS does not directly induce STAT activity in mouse macrophages.. https://doi.org/10.1006/cimm.1996.0129

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