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Sequence, genomic structure, and chromosomal assignment of human DOC-2.

H M Albertsen·S A Smith·R Melis·B Williams·P Holik·J Stevens·R White
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Abstract

DOC-2 is a human gene originally identified as a 767-bp cDNA fragment isolated from normal ovarian epithelial cells by differential display against ovarian carcinoma cells. We have now determined the complete cDNA sequence of the 3.2-kb DOC-2 transcript and localized the gene to chromosome 5. A 12.5-kb genomic fragment at the 5'-end of DOC-2 has also been sequenced, revealing the intron-exon structure of the first eight exons (788 bases) of the DOC-2 gene. Translation of the DOC-2 cDNA predicts a hydrophobic protein of 770 amino acid residues with a molecular weight of 82.5 kDa. Comparison of the DNA and amino acid sequences of DOC-2 to publicly accessible sequence databases revealed 83% identify to p96, a murine protein of similar size, thought to be a mitogen-responsive phosphoprotein. In addition, about 45% identity was observed between the first 140 N-terminal residues of DOC-2 and the Caenorhabditas elegans M110.5 and Drosophila melanogaster Dab genes.

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Adaptor Proteins, Signal TransducingAdaptor Proteins, Vesicular TransportAlternative SplicingAmino Acid SequenceAnimalsApoptosis Regulatory ProteinsBase SequenceCell LineChromosomes, Human, Pair 5DNA, ComplementaryExonsFemaleGenes, Tumor SuppressorHumansIntronsMolecular Sequence DataProteinsRodentiaSequence Homology, Amino AcidTumor Suppressor ProteinsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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H M Albertsen, S A Smith, R Melis, B Williams, P Holik, J Stevens, R White. 1996-04-15. Sequence, genomic structure, and chromosomal assignment of human DOC-2.. https://doi.org/10.1006/geno.1996.0185

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