Rapid detection of mitochondrial sequence polymorphisms using multiplex solid-phase fluorescent minisequencing.

G Tully; K M Sullivan; P Nixon; R E Stones; P Gill

doi:10.1006/geno.1996.0247

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Rapid detection of mitochondrial sequence polymorphisms using multiplex solid-phase fluorescent minisequencing.

G Tully·K M Sullivan·P Nixon·R E Stones·P Gill

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Abstract

This work describes a novel method, multiplex solid-phase fluorescent minisequencing, for the simultaneous detection of several point mutations and/or small deletions and insertions. The method is applied to the analysis of mitochondrial DNA polymorphisms for the purposes of individual identification. A database of 152 British Caucasians and 103 British Afro-Caribbeans has been constructed, and the probability of a chance match between two unrelated individuals is calculated as 0.054 for Caucasians and 0.026 for Afro-Caribbeans.

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Base Sequence Black People DNA Primers DNA, Mitochondrial Fluorescence Genetics, Population Haplotypes Humans Molecular Sequence Data Polymorphism, Genetic Probability Sequence Analysis, DNA United Kingdom White People Journal Article Research Support, Non-U.S. Gov't

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G Tully, K M Sullivan, P Nixon, R E Stones, P Gill. 1996-05-15. Rapid detection of mitochondrial sequence polymorphisms using multiplex solid-phase fluorescent minisequencing.. https://doi.org/10.1006/geno.1996.0247

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