Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.

M J Gait; R C Sheppard

doi:10.1093/nar/4.4.1135

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Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.

M J Gait·R C Sheppard

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Abstract

A method is described that makes use of a new polyamide resin for the rapid synthesis of short oligodeoxyribonucleotides. The method is illustrated by the preparation of two heptadeoxyribonucleotides, d(pT6-C) and d(pC-A-G-T-G-A-T) using a phosphodiester approach. A further development involved use of phenyl isocyanate as an in situ drying agent, which obviated the need for solvent co-evaporation prior tothe internucleotidic coupling steps. Improved fractionation of thymidyl oligonucleotides was obtained by use of a new microparticulate, silica-based anion-exchanger.

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M J Gait, R C Sheppard. 1977. Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.. https://doi.org/10.1093/nar%2F4.4.1135

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