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PubMed · 8662749

Nuclear matrix interactions within the sperm genome.

J A Kramer·S A Krawetz
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Abstract

Analysis of the haploid-expressed human PRM1 --> PRM2 --> TNP2 genic domain has revealed two regions of attachment to the sperm nuclear matrix. These sperm nuclear matrix attachment regions delimit the DNase I-sensitive domain of this haploid-expressed locus. The domain is intermediately associated with but not attached to the nuclear matrix. DNase I-sensitive genes within the mature sperm nucleus, such as protamine 1, protamine 2, transition protein 2, alpha-globin, and beta-actin, display this intermediate affinity for the sperm nuclear matrix. This may denote their role in templating the male genome prior to fertilization, thus ensuring the formation of a viable male pronucleus during early embryonic development.

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Base SequenceChromosomal Proteins, Non-HistoneChromosome MappingDNAHumansMaleMolecular Sequence DataNuclear MatrixNuclear ProteinsProtaminesSpermatozoaJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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J A Kramer, S A Krawetz. 1996-05-17. Nuclear matrix interactions within the sperm genome.. https://doi.org/10.1074/jbc.271.20.11619

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