Genomic DNA analysis from mouse toe lysates.

N L Nadon; K Draeger

doi:10.1007/bf01969711

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Genomic DNA analysis from mouse toe lysates.

N L Nadon·K Draeger

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Abstract

The protocol described in this report provides a simple, accurate and efficient assay for detection of transgenes and mutations in large colonies of rodents, using crude lysates prepared from the digit cut from the animals for identification purposes. This can be done as early as 6 days of age, minimizing trauma to the mice and allowing assays to be completed long before weaning.

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Animals Base Sequence DNA DNA Mutational Analysis Genome Mice Mice, Jimpy Mice, Mutant Strains Mice, Transgenic Molecular Sequence Data Mutation Myelin Proteolipid Protein Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Toes Transgenes Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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N L Nadon, K Draeger. 1996. Genomic DNA analysis from mouse toe lysates.. https://doi.org/10.1007/bf01969711

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