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PubMed · 8679933

High-concentration ethanol production from cooked corn starch by using medium-temperature cooking process.

Z Chi·J Liu·P Xu
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Abstract

An improved process for high-concentration ethanol production from cooked corn starch by Saccharomyces sp. W4 has been well developed. Simultaneous cooking and liquefaction of corn starch were performed by using thermophilic alpha-amylase at 95 degrees to 105 degrees C. The mash was then saccharified at 60 degrees C by using high-efficiency glucoamylase. Saccharomyces sp. W4 could produce 18.3% (v/v) ethanol at 30 degrees C within 60 hrs, with 1.2% reducing sugar and 4.1% total sugar remaining in the fermented mash. It was found that ammonium sulfate could accelerate the fermentation rate. When 0.9 g of ammonium sulfate was added to 280 mL of the fermentation media, 18.9% (v/v) ethanol could be reached in the mash after 50 hrs of fermentation, leaving 0.27% reducing sugar and 3.1% total sugar in the fermented media.

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Ammonium SulfateCookingEthanolFermentationFood MicrobiologyGlucan 1,4-alpha-GlucosidaseSaccharomycesStarchTemperaturealpha-AmylasesJournal ArticleResearch Support, Non-U.S. Gov't

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Z Chi, J Liu, P Xu. 1995. High-concentration ethanol production from cooked corn starch by using medium-temperature cooking process.. https://pubmed.ncbi.nlm.nih.gov/8679933/

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