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PubMed · 8683563

Soft-X-ray damage to biological samples.

H Fujisaki·S Takahashi·H Ohzeki·K Sugisaki·H Kondo·H Nagata·H Kato·S Ishiwata
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Abstract

X-ray damage to biological samples was investigated in the wavelength region of 2.7-5 nm, which overlaps the so-called 'water-window', the wavelength range of 2.4-4.3 nm usually used in X-ray microscopy. Yeast cells and myofibrils were chosen as representatives of whole cell samples and motile protein systems, respectively. The samples were exposed to X-rays using an apparatus composed mainly of a laser-plasma X-ray source, a Wolter mirror condenser, and a sample cell. The yeast cells lost their dye exclusion ability when the X-ray flux was higher than 1 x 10(6) photons micron-2, while the myofibrils lost contractility when the X-ray flux was higher than 4 x 10(5) photons micron-2. These X-ray fluxes are lower than the flux required for the X-ray microscope observation of biological samples at a resolution higher than that of light microscopes.

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H Fujisaki, S Takahashi, H Ohzeki, K Sugisaki, H Kondo, H Nagata, H Kato, S Ishiwata. 1996. Soft-X-ray damage to biological samples.. https://doi.org/10.1046/j.1365-2818.1996.130412.x

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