The respiration-driven active sodium transport system in E. coli does not function with lithium.

M L Verkhovskaya; M I Verkhovsky; M Wikström

doi:10.1016/0014-5793(96)00531-5

Home/Archive/PubMed

PubMed · 8690090

The respiration-driven active sodium transport system in E. coli does not function with lithium.

M L Verkhovskaya·M I Verkhovsky·M Wikström

View original source ↗Export metadata ↓

Abstract

Comparison of respiration-driven active transport of alkali cations from E. coli cells loaded with Na+ or Li+ showed that Li+ could not be expelled from the cells like Na+. K+ accumulation, which was fast in Na+-loaded cells, was strongly inhibited in Li+-loaded cells, despite high membrane potential and respiratory rate. When Li+-loaded cells were placed into medium containing Na+ instead of Li+, Li+/Na+ exchange took place initially, while K+ accumulation was delayed. Only after almost all inside Li+ was replaced by Na+ did active Na+ and K+ transport commence. These data confirm that it is a distinct active sodium transport system (AST) with Na+,K+/H+ antiporter activity, and not the Na+/H+ antiporters, that is responsible for active Na+ transport in E. coli [Verkhovskaya, M.L., Verkhovsky, M.I. and Wikstrom, M. (1996) Biochim. Biophys. Acta 1273, 207-216]. In contrast to the Na+/H+ antiporters, the AST system is inhibited by Li+.

Explore related subjects

Biological Transport, Active Escherichia coli Lithium Potassium Sodium Journal Article Research Support, Non-U.S. Gov't

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

M L Verkhovskaya, M I Verkhovsky, M Wikström. 1996-06-17. The respiration-driven active sodium transport system in E. coli does not function with lithium.. https://doi.org/10.1016/0014-5793(96)00531-5

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

New insight into the biochemical mechanisms regulating auxin transport in plants.

The transport of the plant hormone auxin has been under intense investigation since its identification 80 years ago. Studies have gradually refined our understanding of the importance of auxin transport in many aspects of plant signalling and development, and the focus has intensified in recent years towards the identification of the proteins involved in auxin transport and their functional mechanism. Within the past 18 months, the field has progressed rapidly, with confirmation that several distinct classes of proteins, previously dubbed as 'putative auxin permeases' or 'auxin transport facilitators', are bona fide transporters of IAA (indol-3-ylacetic acid). In this review we will appraise the recent transport data and highlight likely future research directions, including the characterization of auxiliary proteins necessary for the regulation of auxin transporters.

Biological Transport, Active↗

PubMed2007

Controlling tissue microenvironments: biomimetics, transport phenomena, and reacting systems.

The reconstruction of tissues ex vivo and production of cells capable of maintaining a stable performance for extended time periods in sufficient quantity for synthetic or therapeutic purposes are primary objectives of tissue engineering. The ability to characterize and manipulate the cellular microenvironment is critical for successful implementation of such cell-based bioengineered systems. As a result, knowledge of fundamental biomimetics, transport phenomena, and reaction engineering concepts is essential to system design and development. Once the requirements of a specific tissue microenvironment are understood, the biomimetic system specifications can be identified and a design implemented. Utilization of novel membrane systems that are engineered to possess unique transport and reactive features is one successful approach presented here. The limited availability of tissue or cells for these systems dictates the need for microscale reactors. A capstone illustration based on cellular therapy for type 1 diabetes mellitus via encapsulation techniques is presented as a representative example of this approach, to stress the importance of integrated systems.

Biological Transport, Active↗

PubMed2007

Active sugar transport in health and disease.

Secondary active glucose transport occurs by at least four members of the SLC5 gene family. This review considers the structure and function of two premier members, SGLT1 and SGLT2, and their role in intestinal glucose absorption and renal glucose reabsorption. Genetics disorders of SGLTs include Glucose-Galactose Malabsorption, and Familial Renal Glucosuria. SGLT1 plays a central role in Oral Rehydration Therapy used so effectively to treat secretory diarrhoea such as cholera. Increasing attention is being focused on SGLTs as drug targets for the therapy of diabetes.

Biological Transport, Active↗