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PubMed · 8706152

[High efficiency expression of IL-4 using modified cDNA expression vector in mammalian cells].

Z Wang·C Tian·L Liu·M Xu·J Lu
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Abstract

In order to express eukaryotic gene in eukaryotic cells, a cDNA library was constructed and screened with synthesized probe of IL-4 cDNA. The obtained cDNA fragment 739 bp in length was inserted into pi H3M plasmid and expressed (8 x 10(3) IU/ml) in COS cells. A pdB-HhIL-4 expression plasmid was constructed using pdKCR and pdBPV-1 plasmid and transfected into mouse C127 cells by calcium phosphate coprecipitation. Transfected cells exhibited altered cell morphology, could be continuously cultured in DMEM essential medium and secreted IL-4 lymphokine into 1% FCS condition culture medium. High biological activity (2 x 10(4)/ml) from the culture supernatant was obtained by FACS analysis of Fc epsilon receptor expression on B cell surface.

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AnimalsCells, CulturedCloning, MolecularDNA, ComplementaryDNA, RecombinantEukaryotic CellsFibroblastsGene ExpressionInterleukin-4MicePlasmidsTransfectionEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Z Wang, C Tian, L Liu, M Xu, J Lu. 1995. [High efficiency expression of IL-4 using modified cDNA expression vector in mammalian cells].. https://pubmed.ncbi.nlm.nih.gov/8706152/

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