[Detection of p53 mutation using PCR-SSCP silver staining method].

W Zhao; J Si; K Li; X Zhang; G Song

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PubMed · 8706159

[Detection of p53 mutation using PCR-SSCP silver staining method].

W Zhao·J Si·K Li·X Zhang·G Song

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Abstract

A simple and sensitive technique to detect point mutation of the p53 gene using silver staining method for single-strand conformation polymorphism analysis of polymerase chain reaction (PCR-SSCP) is reported. In this study, the aberrations of the p53 gene (exon 6-8) in clinical specimens of primary cervical carcinoma were examined. Of 27 tumor tissues 2 samples showed mutations of p53 exon 7. Absence of abnormal bands in the p53 exon 6-8 in all ten normal cervical tissues. It appears that inactivation of p53 gene by point mutation is infrequent in clinical samples of human cervical carcinomas.

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Base Sequence DNA, Neoplasm Exons Female Genes, p53 Humans Molecular Sequence Data Point Mutation Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Silver Staining Uterine Cervical Neoplasms English Abstract Journal Article Research Support, Non-U.S. Gov't

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W Zhao, J Si, K Li, X Zhang, G Song. 1995. [Detection of p53 mutation using PCR-SSCP silver staining method].. https://pubmed.ncbi.nlm.nih.gov/8706159/

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