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Changes in M-CSF-like activity during chicken embryonic development.

X Shao·K Kikuchi·E Watari·Y Norose·T Araki·K Yokomuro
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Abstract

We have established a method for separation of chicken bone-marrow cells using Percoll density gradient centrifugation, and have developed a new method for determining chicken M-CSF-like activity employing a liquid culture. Using this method, we determined M-CSF-like activities in egg yolk, chorioallantoic fluid (CAF) and amniotic fluid (AmF), and studied the effects of M-CSF on development of chicken embryos. M-CSF-like activity in egg yolk was at a high level before the incubation of the egg; it began to decrease on the third day of incubation and rapidly decreased on the fourth day, and no significant activity was detected after the tenth day of incubation. M-CSF-like activity in CAF was very low, and it exhibited almost no change during development. No M-CSF-like activity was detected in AmF throughout the experimental period.

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AllantoisAmniotic FluidAnimalsCells, CulturedCentrifugation, Density GradientChick EmbryoChorionColloidsEgg YolkMacrophage Colony-Stimulating FactorPovidoneSilicon DioxideJournal Article

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X Shao, K Kikuchi, E Watari, Y Norose, T Araki, K Yokomuro. 1996. Changes in M-CSF-like activity during chicken embryonic development.. https://doi.org/10.1071/rd9960103

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