[PCR: basics and new developments].

M W Berchtold; U Hübscher

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PubMed · 8720727

[PCR: basics and new developments].

M W Berchtold·U Hübscher

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Abstract

Since its discovery 10 years ago PCR has been introduced for a variety of practical applications. PCR has opened new dimensions particularly in laboratory diagnostics because of its sensitivity, accuracy and speed. In spite of the availability of user friendly kits, basic knowledge is of great importance for the user especially if PCR has to be optimized for special needs or when specific problems arise. The general mechanism of the reaction and the significance of the reaction components and the PCR conditions are discussed initially. Several recent developments in PCR (new enzymes, RNA-PCR, improvements of the specificity, prevention of contamination and development of new equipment) that are critical for the user are shortly introduced. Finally, "long PCR" is discussed in order to demonstrate that even 10 years after the invention of PCR significant new breakthroughs in the PCR technology are still possible.

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Animals Base Sequence Biotechnology DNA Humans Nucleic Acid Conformation Polymerase Chain Reaction Sensitivity and Specificity Comparative Study English Abstract Journal Article Review

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M W Berchtold, U Hübscher. 1996. [PCR: basics and new developments].. https://pubmed.ncbi.nlm.nih.gov/8720727/

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