Apolipoprotein H: a two-step isolation method.

R Gambino; G Ruiu; M Cassader; G Pagano

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PubMed · 8732790

Apolipoprotein H: a two-step isolation method.

R Gambino·G Ruiu·M Cassader·G Pagano

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Abstract

A new method for the purification of apolipoprotein H by affinity chromatography followed by continuous-elution electrophoresis is described. It is both simpler and less complicated than the chromatographic and electrophoretic methods usually used. In addition, apolipoprotein H is isolated in a pure, structurally uncleaved form. This is of importance, as impairment has been detected in commercial preparations. The separation and purification of apolipoprotein H is a necessary prelude to its quantitative determination and phenotyping, and hence the clarification of its physiopathological mechanisms in lipid metabolism.

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Amino Acid Sequence Apolipoproteins Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Glycoproteins Humans Lipid Metabolism Molecular Sequence Data beta 2-Glycoprotein I Journal Article Research Support, Non-U.S. Gov't

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R Gambino, G Ruiu, M Cassader, G Pagano. 1996. Apolipoprotein H: a two-step isolation method.. https://pubmed.ncbi.nlm.nih.gov/8732790/

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