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Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring.

A Ogura·J Matsuda·T Asano·O Suzuki·R Yanagimachi
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Abstract

PURPOSE: This study was performed to determine whether frozen-thawed mouse round spermatids can fertilize oocytes and contribute to normal embryo development. METHODS: Freshly collected mouse testicular cells were frozen in PBS containing 7.5% glycerol and 7.5% fetal bovine serum. After thawing and removal of the cryoprotectants, round spermatids were selected and injected individually into mature oocytes which had been previously activated with Sr(2+)-containing Ca(2+)-free medium. RESULTS: After thawing, 75-85% of testicular cells were alive. About 90% of the oocytes were fertilized by intracytoplasmic injection of frozen-thawed round spermatids; 11% (17/150) of embryos transferred to foster mothers developed into normal offspring. CONCLUSIONS: Mouse round spermatids can be cryopreserved for production of normal offspring.

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AnimalsCryopreservationEmbryo TransferEmbryonic and Fetal DevelopmentFemaleFertilization in VitroMaleMiceMice, Inbred C57BLMice, Inbred DBAOocytesSperm-Ovum InteractionsSpermatidsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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A Ogura, J Matsuda, T Asano, O Suzuki, R Yanagimachi. 1996. Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring.. https://doi.org/10.1007/bf02066177

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