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Immunochemical methods for quantification of apolipoprotein A-IV.

N H Fidge
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Abstract

Several methods are available for the immunoassay of apoA-IV levels in plasma, or lipoproteins. The method of choice depends on the question being asked. If sensitivity is not a major determinant, simple immunoelectrophoresis is probably sufficient. To determine apoA-IV levels in plasma or lipoprotein fractions, either radioimmunoassay or a competitive ELISA is indicated. The competitive ELISA described above, however, offers sensitivity as well as rapidity and case of performance. When very low levels of apoA-IV are present (such as those produced by cultured cells), the higher sensitivity of the sandwich ELISA may be required.

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AnimalsApolipoproteins AChromatography, GelChromatography, High Pressure LiquidChylomicronsElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayHumansImmune SeraImmunoassayImmunoelectrophoresisLipid MetabolismRadioimmunoassayRatsJournal Article

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BibTeXRIS

N H Fidge. 1996. Immunochemical methods for quantification of apolipoprotein A-IV.. https://doi.org/10.1016/s0076-6879(96)63021-3

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