Selected locus and multiple panel models for radiation hybrid mapping.

K L Lunetta; M Boehnke; K Lange; D R Cox

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PubMed · 8751873

Selected locus and multiple panel models for radiation hybrid mapping.

K L Lunetta·M Boehnke·K Lange·D R Cox

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Abstract

We develop two new types of models for whole-genome radiation hybrid mapping using the general multipoint framework. The first, selected locus models, are appropriate for mapping markers in the region of a selectable locus that was used in creation of the hybrids. The models allow for strong retention of the selectable locus, with retention rates decreasing with increasing distance from the selectable locus in both directions. We illustrate the application of these models with 10 chromosome 17 sequence-tagged site (STS) markers and the thymidine kinase (TK) locus typed on a whole-genome hybrid panel in which TK was used in the selection process. The second set of models are appropriate when loci typed on two or more independent panels are to be used to build maps. Maps can be built assuming interlocus distances are independent or proportional between the panels, and the hypothesis of proportional distances can be tested. We illustrate the application of these models by using 27 chromosome 21 STS markers typed on two hybrid panels created with radiation doses of approximately 10,000 and approximately 50,000 Rads.

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Chromosome Mapping Chromosomes, Human, Pair 17 Chromosomes, Human, Pair 21 Genetic Markers Genome, Human Humans Hybrid Cells Models, Genetic Models, Statistical Multivariate Analysis Polyploidy Sequence Tagged Sites Journal Article Research Support, U.S. Gov't, P.H.S.

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K L Lunetta, M Boehnke, K Lange, D R Cox. 1996. Selected locus and multiple panel models for radiation hybrid mapping.. https://pubmed.ncbi.nlm.nih.gov/8751873/

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