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PubMed · 8752097

Three dynamin-encoding genes are differentially expressed in developing rat brain.

T Cook·K Mesa·R Urrutia
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Abstract

Dynamin proteins are members of a recently described family of GTPases involved in receptor-mediated processes. To date, three different dynamin-encoding genes have been identified in mammalian tissues. Dynamin I is expressed only in neurons, whereas dynamin II is ubiquitously expressed. A third isoform, dynamin III, was originally isolated from a rat testis cDNA library and shown to be testis-specific. However, here we report the cloning and characterization of dynamin III from brain and lung, demonstrating a more extended pattern of expression for this isoform. In addition, we have investigated the temporal pattern of expression of these three genes during brain development. We find that both dynamin I and dynamin III mRNA levels are up-regulated during embryogenesis, whereas dynamin II mRNA levels remain unchanged. From these results, we conclude that dynamin III is not a testis-specific isoform and, furthermore, that rat brain expresses three different dynamin-encoding genes that are differentially regulated during development. Therefore, this large isoform diversity of dynamin proteins in brain predicts a significant complexity in the understanding of dynamin-based processes in this tissue.

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Alternative SplicingAmino Acid SequenceAnimalsBrainDynamin IDynamin IIIDynaminsEndocytosisGTP PhosphohydrolasesGene Expression Regulation, DevelopmentalGene LibraryGenetic TestingIsoenzymesLungMaleMicrotubulesMolecular Sequence DataPC12 CellsRNA, MessengerRatsRats, Sprague-DawleyTestisTime FactorsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T Cook, K Mesa, R Urrutia. 1996. Three dynamin-encoding genes are differentially expressed in developing rat brain.. https://doi.org/10.1046/j.1471-4159.1996.67030927.x

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