Alternative processing of the sarco/endoplasmic reticulum Ca(2+)-ATPase transcripts during muscle differentiation is a specifically regulated process.

L Van den Bosch; L Mertens; Y Cavaloc; M Peterson; F Wuytack; J Eggermont

doi:10.1042/bj3170647

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PubMed · 8760345

Alternative processing of the sarco/endoplasmic reticulum Ca(2+)-ATPase transcripts during muscle differentiation is a specifically regulated process.

L Van den Bosch·L Mertens·Y Cavaloc·M Peterson·F Wuytack·J Eggermont

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Abstract

Expression of the muscle-specific 2a isoform of the sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA2) requires activation of an otherwise inefficient splice process at the 3'-end of the primary gene transcript. We provide evidence that SERCA2 splicing is a specifically regulated process, rather than the result of an increase in general splice efficiency or a decrease in polyadenylation efficiency at the 5'-most polyadenylation site. This is indicated by the fact that changes in general splice and polyadenylation efficiency, as observed during B-cell maturation, did not affect SERCA2 splicing. Furthermore, expression and overexpression studies did not support the hypothesis that changes in the level of the alternative splice factor ASF/SF2 or other arginine and serine rich proteins are sufficient to obtain the regulation of muscle- and neuronal-specific splicing.

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Alternative Splicing Animals Calcium-Transporting ATPases Cell Differentiation Endoplasmic Reticulum Mice Muscles Nuclear Proteins RNA-Binding Proteins Sarcoplasmic Reticulum Serine-Arginine Splicing Factors Journal Article Research Support, Non-U.S. Gov't

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L Van den Bosch, L Mertens, Y Cavaloc, M Peterson, F Wuytack, J Eggermont. 1996-08-01. Alternative processing of the sarco/endoplasmic reticulum Ca(2+)-ATPase transcripts during muscle differentiation is a specifically regulated process.. https://doi.org/10.1042/bj3170647

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