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Postelectrophoresis capillary scanning method for DNA sequencing.

S Kim·H J Yoo·J H Hahn
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Abstract

A postelectrophoresis capillary scanning method for increasing the throughput of DNA sequencing has been developed. The method features a spatially and temporally separated arrangement between capillary gel electrophoresis separation of DNA sequencing fragments and the visualization of the separation pattern. Fluroescently labeled DNA sequencing fragments of pBluescript SK(-) were produced in enzymatic chain-termination reactions and separated by capillary gel electrophoresis using a 110 cm fused silica capillary with a transparent polymer coating. A partial sequence from the DNA was then determined by repeated scanning of the capillary at a rate of 8.39 cm/s, with changing excitation wavelengths and spectral filter sets.

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Base SequenceElectrophoresis, CapillaryMolecular Sequence DataSequence Analysis, DNAJournal ArticleResearch Support, Non-U.S. Gov't

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S Kim, H J Yoo, J H Hahn. 1996-03-01. Postelectrophoresis capillary scanning method for DNA sequencing.. https://doi.org/10.1021/ac9508107

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