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Simple method for detecting fluconazole-resistant yeasts with chromogenic agar.

T F Patterson·S G Revankar·W R Kirkpatrick·O Dib·A W Fothergill·S W Redding·D A Sutton·M G Rinaldi
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Abstract

A method for detecting fluconazole-resistant yeasts was developed that uses chromogenic agar containing fluconazole. Yeasts were plated on media with fluconazole at 0, 8, and 16 micrograms/ml. On media without fluconazole, normal growth of susceptible yeasts (defined as those having a fluconazole MIC of < 8 micrograms/ml) was detected, while fluconazole-containing media suppressed susceptible strains and normal colonies of resistant yeasts (fluconazole MICs of > or = 8 micrograms/ml) were detected. This method was used to screen for resistance in oropharyngeal candidiasis. Isolates having fluconazole MICs of > or = 8 micrograms/ml and < 8 micrograms/ml were correctly predicted in 43 of 45 cultures and 115 of 116 cultures, respectively. This screening method appears to be rapid and sensitive for detection of fluconazole-resistant yeasts.

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AgarAntifungal AgentsCandidaCandida albicansCandidiasis, OralChromogenic CompoundsCulture MediaDrug Resistance, MicrobialEvaluation Studies as TopicFluconazoleHumansMycologySensitivity and SpecificityYeastsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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T F Patterson, S G Revankar, W R Kirkpatrick, O Dib, A W Fothergill, S W Redding, D A Sutton, M G Rinaldi. 1996. Simple method for detecting fluconazole-resistant yeasts with chromogenic agar.. https://doi.org/10.1128/jcm.34.7.1794-1797.1996

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