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PubMed · 8790710

Recent progress in oligonucleotide synthesis.

W Pfleiderer·S Matysiak·F Bergmann·R Schnell
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Abstract

New blocking group combinations for the machine-aided oligoribonucleotide synthesis on solid phase material have been developed and tested regarding their general application. An acetal function for 2'-OH protection offers a series of advantages in the synthetic approach but special conditions have to be fulfilled in order to guarantee a selective cleavage of the temporary 5'-OH blocking group such as the dansylethoxycarbonyl or even the acid-labile dimethoxytrityl group in the chain elongation process. The final removal of the 2'-O-acetal function in the partially deblocked oligomer proceeds unexpectedly well under weak acidic conditions due to a supposed intramolecular acid catalysis.

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AcetalsDansyl CompoundsIndicators and ReagentsMolecular StructureOligodeoxyribonucleotidesOligonucleotidesOligoribonucleotidesJournal ArticleReview

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BibTeXRIS

W Pfleiderer, S Matysiak, F Bergmann, R Schnell. 1996. Recent progress in oligonucleotide synthesis.. https://pubmed.ncbi.nlm.nih.gov/8790710/

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