Endothelial cell associated anti-elastolytic activity.

M E Hanley; L S Terada; J C Cheronis; J E Repine

doi:10.1007/bf01488207

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Endothelial cell associated anti-elastolytic activity.

M E Hanley·L S Terada·J C Cheronis·J E Repine

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Abstract

Addition of cultured and then carefully-washed bovine pulmonary artery endothelial cells (EC) decreased (p < 0.05) human neutrophil elastase activity (HNE) in vitro. HNE activity was also decreased (p < 0.05) by addition of histone or protamine treated EC. However, addition of papain or trypsin treated EC decreased HNE activity less than addition of untreated cells suggesting that a protein rather than a difference in cell surface charge was responsible. Other observations suggest that EC anti-elastolytic activity was not due to binding of antiprotease from culture media but was dependent on EC protein synthesis. First, addition of EC grown previously in serum-free media decreased HNE activity the same (p < 0.05) as addition of EC cultured in media containing serum. Second, addition of EC treated beforehand with cycloheximide decreased HNE activity less than (p < 0.05) addition of untreated control EC. We conclude that EC most likely make and have anti-elastolytic activity on their surfaces and speculate that EC associated anti-elastolytic activity may modulate inflammatory, repair and other biologic processes involving neutrophil elastase.

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Animals Cattle Cells, Cultured Endothelium, Vascular Enzyme Inhibitors Humans Leukocyte Elastase Substrate Specificity Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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M E Hanley, L S Terada, J C Cheronis, J E Repine. 1996. Endothelial cell associated anti-elastolytic activity.. https://doi.org/10.1007/bf01488207

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