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PubMed · 8799837

Accelerated neuronal differentiation induced by p53 suppression.

A Ferreira·K S Kosik
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Abstract

p53, a tumor suppressor gene product, has been implicated in the control of cell growth and malignant transformation in different cell types. Here we studied the role of p53 in normal central nervous system development. We show that p53 is expressed in neuroblasts and is down regulated when migrating neurons reach their destination. The suppression of p53 either by the addition of antisense oligonucleotides to culture medium or by the culture of neurons from p53-/- mice accelerated their differentiation. This effect is accompanied by an early induction of MAP1b and a premature dephosphorylation of tau. p53 suppression also reduced levels of p21. Taken collectively these results suggest that the expression of p53 in neuroblasts might prevent neuronal terminal differentiation.

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AnimalsBase SequenceCell DifferentiationCyclin-Dependent KinasesDown-RegulationGene Expression Regulation, DevelopmentalGenes, p53ImmunohistochemistryMiceMice, KnockoutMice, TransgenicMicrotubule-Associated ProteinsMicrotubulesNeuronsOligonucleotides, AntisensePhenotypeRatsTumor Suppressor Protein p53Journal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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A Ferreira, K S Kosik. 1996. Accelerated neuronal differentiation induced by p53 suppression.. https://doi.org/10.1242/jcs.109.6.1509

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