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PubMed · 8819831

Complex interaction between different proteinaceous components within the cell-wall structure of Candida albicans.

J L Lopez-Ribot·D A Cortlandt·D C Straus·K J Morrow·W L Chaffin
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Abstract

We have previously described a monoclonal antibody, MAb DC3:H10, which recognized an epitope preferentially expressed on the surface of Candida albicans germ tubes. In the present study we examined the MAb-reactive material further. Immunoblot analysis of the material purified partially by Sephadex G-200 and DEAE-Sephacel chromatography reacted with antibodies to the C. albicans C3d receptor (CR2). In an ELISA, MAb DC3:H10 captured antigen that was recognized by both anti-CR2 and anti-mp58 fibrinogen binding mannoprotein polyclonal antibodies. The MAb DC3:H10 failed to compete with either of these antisera in an ELISA. Indirect immunofluorescence (IIF) analysis showed differences in surface distribution for the MAb DC3:H10, the CR2, and the mp 58 epitopes. Dual labeling IIF experiments showed MAb DC3:H10 binding to be unaffected by binding of fibrinogen or anti-mp58 antibody. However, the binding patterns of MAb DC3:H10 were modified in the presence of anti-CR2 antibody, suggesting a complex interaction between these cell wall components.

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Antibodies, FungalAntibodies, MonoclonalCandida albicansEnzyme-Linked Immunosorbent AssayFluorescent Antibody TechniqueFungal ProteinsImmunoblottingMacromolecular SubstancesMembrane GlycoproteinsProtein BindingReceptors, Complement 3dComparative StudyJournal Article

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J L Lopez-Ribot, D A Cortlandt, D C Straus, K J Morrow, W L Chaffin. 1995. Complex interaction between different proteinaceous components within the cell-wall structure of Candida albicans.. https://doi.org/10.1007/bf01103780

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