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PubMed · 8825629

Single-base sequencing and similarity comparisons.

G Xie·R Lobb·W J Bruno·D C Torney·J M Gatewood
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Abstract

A "single-base sequence" is a DNA sequence in which the identities and locations of bases of only one type have been determined. We present experimental procedures for single-base sequencing and describe the effective use of existing software (FASTA) in similarity comparisons of single-base sequences. We determined the theoretical and experimental minimum sequence lengths required for identification of a sequence within a large dataset and optimized the FASTA parameters for use in single-base similarity comparisons. Single-base sequences have been used to identify cDNAs occurring in a database. Single-base sequencing could be used to reduce the redundancy of "shot-gun sequencing."

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G Xie, R Lobb, W J Bruno, D C Torney, J M Gatewood. 1995-12-10. Single-base sequencing and similarity comparisons.. https://doi.org/10.1006/geno.1995.1263

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