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Atomic force microscopy proposes a novel model for stem-loop structure that binds a heat shock protein in the Staphylococcus aureus HSP70 operon.

T Ohta·S Nettikadan·F Tokumasu·H Ideno·Y Abe·M Kuroda·H Hayashi·K Takeyasu
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Abstract

The Staphylococcus aureus HSP70 operon produces a polycistronic RNA in response to heat shock, and ORF37 is the first protein to be translated. The promoter of this operon contains a palindromic nucleotide sequence that may form a stem-loop structure. Structural analysis of the promoter regions by atomic force microscopy (AFM) revealed a quadruplet that consists of a pair of stem-loops. A novel "SL2S' (Stem-Loop-Loop-Stem) model was proposed for this structure. AFM also revealed the binding of ORF37 to the quadruplet, establishing a molecular mechanism for this heat shock gene expression; ORF37 acts as a regulator by binding to the SL2S structure in the promoter.

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Base SequenceDNA, BacterialGenes, BacterialHSP70 Heat-Shock ProteinsMicroscopy, Atomic ForceModels, StructuralMolecular Sequence DataNucleic Acid ConformationOpen Reading FramesOperonRNA, BacterialRestriction MappingStaphylococcus aureusJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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T Ohta, S Nettikadan, F Tokumasu, H Ideno, Y Abe, M Kuroda, H Hayashi, K Takeyasu. 1996-09-24. Atomic force microscopy proposes a novel model for stem-loop structure that binds a heat shock protein in the Staphylococcus aureus HSP70 operon.. https://doi.org/10.1006/bbrc.1996.1421

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