PubMed · 8832441
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O A Mamer. 1996-06-28. Stable isotope dilution techniques.. https://doi.org/10.1016/0378-4347(96)00055-2
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The advent of gene therapy and polynucleotide-based vaccines has resulted in the use of plasmid DNA as a drug substance. Although biologically (cell or animal) based assays must currently be employed to establish the identity and potency of such drugs, we argue that in the future, a combination of microchip-based mutation detection devices combined with an array of chromatographic, electrophoretic, hydrodynamic, and spectroscopic methods can be employed to rigorously establish these properties. We review a variety of such methods in this context and also consider the issue of the chemical stability of plasmids. Extensive comparison is made to protein-based pharmaceuticals with the unique importance of polynucleotide sequence emphasized in comparison to protein tertiary structure.
The electrokinetic molecular effect known as electromolecular propulsion (EMP) was used to examine a variety of commonly used dyes that are used as biological stains. The dyes were electrokinetically mobilized using several organic solvent mixtures on a variety of thin layer substrates. Analysis was completed in seconds to minutes. Nearly all dyes tested separated into multiple colored components. The solvents and substrates used were well suited for qualitatively analyzing a broad variety of hydrophilic and lipophilic colorants. Speed, resolving power and operational simplicity of this technique make it convenient for effectively "fingerprinting" for consistency in dye products. EMP may be broadly applied to study quality control of dyes and their stains. Components not ordinarily known to be present can be readily detected using this technology. EMP can complement or offer a versatile alternative to existing analytical methods.