Lymphocyte subsets in multiple sclerosis. A study with two-colour fluorescence analysis.

P Bongioanni; C Fioretti; R Vanacore; F Bianchi; F Lombardo; F Ambrogi; G Meucci

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PubMed · 8836975

Lymphocyte subsets in multiple sclerosis. A study with two-colour fluorescence analysis.

P Bongioanni·C Fioretti·R Vanacore·F Bianchi·F Lombardo·F Ambrogi·G Meucci

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Abstract

Multiple sclerosis (MS) is postulated to be an immunopathologically mediated disease. This concept is supported by the finding of abnormally distributed peripheral blood T-cell subsets and a decreased T-suppressor function. Thirty-seven MS patients have been selected according to the criteria for definite MS. Fluorescein- or phycoerythrin-conjugated monoclonal antibodies have been used to define different lymphocyte subsets: CD4+, CD5+, CD8+, CD19+, CD38+, CD45RA+, CD4+CD45RA+, CD19+CD5+, CD8+CD38+. In relapsing-remitting (RR)-MS patients a significantly decreased percentage of CD19+ cells and in progressive MS patients a significantly increased percentage of CD19+CD5+ cells have been found. During a relapse in RR-MS, a significantly decreased percentage of CD4+CD45RA+ cells and a significantly increased percentage of CD8+CD38+ cells have been observed. Moreover, in RR-MS patients a significantly increased percentage of CD38+ cells and significantly high IgM amounts have been found. The increased percentage of CD19+CD5+ and CD38+ cells (together with high IgM levels) and the reduced percentage of CD4+CD45RA+ lymphocytes could be related to an activation of both cellular and humoral immune response in acute MS.

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Adolescent Adult Age of Onset Antibodies, Monoclonal Antigens, CD Female Humans Male Middle Aged Multiple Sclerosis Recurrence Reference Values T-Lymphocyte Subsets T-Lymphocytes, Regulatory Journal Article

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P Bongioanni, C Fioretti, R Vanacore, F Bianchi, F Lombardo, F Ambrogi, G Meucci. 1996. Lymphocyte subsets in multiple sclerosis. A study with two-colour fluorescence analysis.. https://pubmed.ncbi.nlm.nih.gov/8836975/

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