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PubMed · 8844707

E-selectin expression on human brain microvascular endothelial cells.

D C Hess·Y Thompson·A Sprinkle·J Carroll·J Smith
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Abstract

E-Selectin is an endothelial adhesion molecule involved in binding and targeting of neutrophils. Little is known of its expression in the brain. We examined the expression of E-selectin on cultured human brain microvascular endothelial cells (HBMEC). There was no basal expression of E-selectin on HBMEC but with I1-1b, tumor necrosis factor (TNF), or lipopolysaccharide (LPS) stimulation there was surface expression at 4 h. The expression was quantitatively less than on cultured human umbilical vein endothelial cells (HUVEC). The cytokine-induced upregulation was partially inhibited with the glutathione donor, N-acetylcysteine (NAC), the free radical scavenger, dimethylthiourea (DMTU; 15 mM) and dexamethasone (1 microM). Allopurinol (100 microM) had no effect. TNF activated nuclear factor kappa B (NF kappa B) in HBMEC. This activation could be attenuated by prior treatment with NAC and dexamethasone. Thiol donors and corticosteroids could play a role in inhibiting potentially deleterious neutrophil-endothelial interactions in inflammatory conditions involving the brain.

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AllopurinolCells, CulturedDexamethasoneE-SelectinElectrophoresisEndothelium, VascularEnzyme InhibitorsGlucocorticoidsHumansInterleukin-1LipopolysaccharidesMicrocirculationNF-kappa BTemporal LobeTumor Necrosis Factor-alphaUmbilical VeinsUp-RegulationComparative StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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D C Hess, Y Thompson, A Sprinkle, J Carroll, J Smith. 1996-07-26. E-selectin expression on human brain microvascular endothelial cells.. https://doi.org/10.1016/0304-3940(96)12837-8

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