PubMed HealthSearch

PubMed · 8857573

Thin-layer chromatography: a neglected technique.

Abstract

Thin-layer (or planar) chromatography (TLC) is critically reviewed from the point of view of drug analysis in biological fluids. The capabilities of the various techniques of TLC are described and their advantages and disadvantages are discussed. An example of the use of high-performance TLC with scanning densitometry for the quantitative determination of antipyrine in human plasma is provided. The use of TLC-mass spectrometry and TLC-tandem mass spectrometry, directly from the sorbent, in the identification of the compounds separated by TLC is discussed.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

I D Wilson. 1996. Thin-layer chromatography: a neglected technique.. https://doi.org/10.1097/00007691-199608000-00030

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Ricin RCA60: evidence of its phospholipase activity.

The present work documents, on a qualitative and quantitative basis, the lipolytic activity of ricin protein RCA60 on glycerophospholipids. RCA60 demonstrates a low level of hydrolysis towards radioactive dipalmitoyl-glycerophosphatidylcholine. This observation was confirmed on a better substrate, palmitoyl-oleoyl-glycerophosphatidylcholine, after analysis of the reaction products by thin-layer and gas chromatography. A comparable hydrolytic activity was observed when palmitoyl-oleoyl-glycerophosphatidylethanolamine was used as substrate. The nature of the hydrolysis products supports the conclusion that RCA60 demonstrates phospholipase A1 and A2 activities as well as a lysophospholipase activity of A1 and A2 type. The insensitivity of this lipolytic activity towards calcium ions and the presence of the already described consensus sequence of lipases, Gly-Xaa-Ser-Xaa-Gly, in the primary sequence of the B-chain of RCA60 support the idea that the lipolytic activity of RCA60 is more related to the lipase family than to the phospholipases A. We hypothesize that such activity contributes to the mechanism which underlies the expression of the cytotoxicity of RCA60.

Chromatography, Thin Layer

Solid phase synthesis of structurally diverse pyrimido[4,5-d] pyrimidines for the potential use in combinatorial chemistry.

An efficient solid phase synthesis of pyrimido[4,5-d]pyrimidine derivatives is described. Reaction of polymer-bound pyrimidine 1 with urea or thiourea followed by cleavage from the support provided 4-aminopyrimido[4,5-d]pyrimidines 4 and 5 while treatment of 6 with phenyl isocyanate or phenyl isothiocyanate followed by cleavage from resin afforded 3-phenylpyrimido[4,5-d]pyrimidines 9 and 10.

Chromatography, Thin Layer

Synthesis of 6-exomethylenepenams as beta-lactamase inhibitors.

The 6,6-dibromopenam (6) was treated with CH3MgBr and carbaldehyde 5 to afford the hydroxy compound 7, which was reacted with acetic anhydride to give acetoxy compound 8. The deacetobromination of 8 with zinc and acetic acid gave 6-exomethylenepenams, E-isomer 10 and Z-isomer 9, which was oxidized to sulfone 11 by m-CPBA. The p-methoxybenzyl compounds were deprotected by AlCl3 and neutralized to give the sodium salts 12, 13 and 14.

Chromatography, Thin Layer