New rapid microbiological diagnostic techniques.

D Burgner; D Isaacs; R Givney

doi:10.1111/j.1440-1754.1996.tb00900.x

Home/Archive/PubMed

PubMed · 8860378

New rapid microbiological diagnostic techniques.

D Burgner·D Isaacs·R Givney

View original source ↗Export metadata ↓

Abstract

OBJECTIVE: To briefly and critically review the role of new, rapid techniques, especially PCR, in the diagnosis of infectious diseases. METHODOLOGY: Review of the literature. RESULTS: New diagnostic techniques show great promise but many techniques are still in their infancy and the relative roles of the various methods remain uncertain. CONCLUSIONS: It seems likely that different techniques will continue to be useful in a number of different contexts. No one technique is likely to become 'diagnostically dominant' in the foreseeable future.

Explore related subjects

Enzyme-Linked Immunosorbent Assay Humans Microbiological Techniques Polymerase Chain Reaction Serologic Tests Time Factors Virus Cultivation Journal Article Review

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

D Burgner, D Isaacs, R Givney. 1996. New rapid microbiological diagnostic techniques.. https://doi.org/10.1111/j.1440-1754.1996.tb00900.x

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

Recombinant truncated nucleocapsid protein as antigen in a novel immunoglobulin M capture enzyme-linked immunosorbent assay for diagnosis of severe acute respiratory syndrome coronavirus infection.

We report the development of an immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for severe acute respiratory syndrome coronavirus (SARS-CoV) by using recombinant truncated SARS-CoV nucleocapsid protein as the antigen. The newly developed MAC-ELISA had a specificity and sensitivity of 100% as evaluated by using sera from healthy volunteers and patients with laboratory-confirmed SARS. Using serial serum samples collected from SARS patients, the times to seroconversion were determined by IgM antibody detection after SARS-CoV infection. The median time to seroconversion detection was 8 days (range, 5 to 17 days) after disease onset, and the seroconversion rates after the onset of illness were 33% by the first week, 97% by the second week, and 100% by the third week. Compared with the results of our previous report on the detection of IgG, the median seroconversion time by IgM detection was 3 days earlier and the seroconversion rate by the second week after the illness for IgM was significantly higher than by IgG assay. Our results indicating that the IgM response appears earlier than IgG after SARS-CoV infection in consistent with those for other pathogens. Our newly developed MAC-ELISA system offers a new alternative for the confirmation of SARS-CoV infection.

Enzyme-Linked Immunosorbent Assay↗

PubMed2007

Specificity of enzyme-linked immunosorbent assay for IgG anti-NR2 glutamate receptor antibodies: comment on the concise communication by Yoshio et al.

Explore the source record for details and available documents.

Enzyme-Linked Immunosorbent Assay↗

PubMed2006

Perfusion with lipopolysaccharide differently affects the secretion of tumor necrosis factor-alpha and interleukin-6 by term and preterm human placenta.

This study has compared the functional capacity of term and preterm placentas in terms of production of pro-inflammatory cytokines in a perfusion system reflecting their ability to react to inflammatory agents, such as lipopolysaccharide (LPS), mimicking the situation of chorioamnionitis. We have demonstrated that term placentas secrete higher levels of tumor necrosis factor (TNF)-alpha compared with preterm placentas. Moreover, TNF-alpha secretion was significantly higher after exposure to LPS in the maternal and fetal sides of term placentas. In contrast, in preterm placentas, only the fetal side responded with a significant increase in secretion of TNF-alpha after exposure to LPS. The maternal side of term placentas secreted significantly higher amounts of interleukin (IL)-6 compared with preterm placentas. Exposure to LPS significantly decreased IL-6 secretion from the maternal side in both term and preterm placentas. Moreover, the fetal side of term placentas secreted significantly lower amounts of IL-6 compared with preterm placentas. In summary, this study has indicated that term and preterm placental tissues have a differing responsiveness to LPS stimulation, with term placentas disposed to a higher TNF-alpha:IL-6 ratio. Release of cytokines into fetal circulation is less than into the maternal side. However, TNF-alpha is released into fetal circulation after LPS stimulation and this may be relevant to the etiology of chorioamnionitis.

Enzyme-Linked Immunosorbent Assay↗