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Expression of human foamy virus reverse transcriptase involves a spliced pol mRNA.

I Jordan·J Enssle·E Güttler·B Mauer·A Rethwilm
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Abstract

In human foamy virus (HFV) the reverse transcriptase is expressed independently of the Gag protein as a 127-kDa Pol precursor molecule. Evaluating the mechanism of Pol expression we identified a spliced mRNA which uses the main 5' splice donor and a splice acceptor site located in the gag gene. The significance of this spliced transcript for HFV Pol expression was studied by constructing a virus with a mutated splice acceptor site. This virus was unable to express detectable Pol proteins after transient transfection. Replication of the mutant was studied by a sensitive assay based on HFV transactivator-stimulated expression of an integrated lacZ gene under control of the HFV long terminal repeat. Whereas in the first 2 weeks after transfection the mutant replicated 3 to 5 order of magnitude less well than wild-type virus, extracellular titers obtained thereafter were similar to those of wild-type virus. This increase in replication competence was accompanied by a reversion of the mutated splice acceptor site. The results underlined the importance of the spliced pol transcript for HFV replication and pointed to a second mechanism of Pol expression. Indicator gene assays suggest that this other mechanism is likely to be a transactivator-dependent cryptic promoter in the gag gene which gives rise to Pol-encoding transcripts.

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AnimalsCell LineCricetinaeGene ExpressionGene Products, polHumansProtein PrecursorsRNA SplicingRNA, MessengerRNA-Directed DNA PolymeraseSpumavirusJournal ArticleResearch Support, Non-U.S. Gov't

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I Jordan, J Enssle, E Güttler, B Mauer, A Rethwilm. 1996-10-01. Expression of human foamy virus reverse transcriptase involves a spliced pol mRNA.. https://doi.org/10.1006/viro.1996.0534

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