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Hyperresponsive B cells in CD22-deficient mice.

T L O'Keefe·G T Williams·S L Davies·M S Neuberger
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Abstract

CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. Splenic B cells from mice with a disrupted CD22 gene were found to be hyperresponsive to receptor signaling: Heightened calcium fluxes and cell proliferation were obtained at lower ligand concentrations. The mice gave an augmented immune response, had an expanded peritoneal B-1 cell population, and contained increased serum titers of autoantibody. Thus, CD22 is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering.

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AnimalsAntibodies, AntinuclearAntibody FormationAntigens, CDAntigens, Differentiation, B-LymphocyteB-LymphocytesCalciumCell Adhesion MoleculesFemaleGene TargetingImmunizationImmunoglobulin MImmunophenotypingLectinsLymphocyte ActivationMaleMiceMice, Inbred BALB CMice, Inbred C57BLPhosphorylationReceptors, Antigen, B-CellSialic Acid Binding Ig-like Lectin 2Signal TransductionTransfectionJournal ArticleResearch Support, Non-U.S. Gov't

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T L O'Keefe, G T Williams, S L Davies, M S Neuberger. 1996-11-01. Hyperresponsive B cells in CD22-deficient mice.. https://doi.org/10.1126/science.274.5288.798

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