Extracellular Ca2+ changes during transmitter application in the leech central nervous system.

C Lohr; C R Rose; J W Deitmer

doi:10.1016/0304-3940(96)12371-5

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Extracellular Ca2+ changes during transmitter application in the leech central nervous system.

C Lohr·C R Rose·J W Deitmer

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Abstract

Changes in extracellular Ca2+ concentration ([Ca2+]e) evoked by transmitters and transmitter agonists, respectively, and by elevation of bath K+ concentration were recorded in isolated segmental ganglia of the leech Hirudo medicinalis using Ca(2+)-selective microelectrodes. A 1-min bath application of kainate (10 microM), glutamate (1 mM), aspartate (1 mM), or carbachol (200 microM) decreased [Ca2+]e by up to 1 mM, whereas the inhibitory transmitters gamma-amino butyric acid (GABA, 100 microM) and serotonin (5-HT, 100 microM) did not change [Ca2+]e. The amplitude of the kainate-induced changes in [Ca2+]e increased with repetitive applications, and changes were blocked by 6-cyano-7-dinitroquinozaline-2,3-dione (CNQX). Elevation of bath K+ concentration from 4 to 40 mM led to a Ni(2+)-sensitive decrease in [Ca2+]e by 0.9 mM. Our results suggest that excitatory transmission in the leech central nervous system might be accompanied by substantial decreases in [Ca2+]e.

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Animals Calcium Carbachol Central Nervous System Ganglia Glutamic Acid Kainic Acid Leeches Neurotransmitter Agents Potassium Journal Article Research Support, Non-U.S. Gov't

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C Lohr, C R Rose, J W Deitmer. 1996-02-16. Extracellular Ca2+ changes during transmitter application in the leech central nervous system.. https://doi.org/10.1016/0304-3940(96)12371-5

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