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PubMed · 8871570

Gene transfer mediated by alpha2-macroglobulin.

H Schneider·K Huse·G Birkenmeier·A Otto·G H Scholz
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Abstract

alpha2-Macroglobulin covalently linked to poly(L)-lysine can be used as a vehicle for receptor-mediated gene transfer. This modified alpha2-macroglobulin maintains its ability to bind to the alpha2-macroglobulin receptor, and was shown to introduce a luciferase reporter gene plasmid into HepG2 human hepatoma cells in vitro. The alpha2-macroglobulin receptor is a very large and multifunctional cell surface receptor, whose rapid and efficient internalization rate makes it attractive for gene therapy, e.g. for hepatic gene targeting via injection into the portal vein.

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Gene Transfer TechniquesGenes, ReporterHumansLow Density Lipoprotein Receptor-Related Protein-1LuciferasesPolylysineReceptors, ImmunologicTumor Cells, Culturedalpha-MacroglobulinsJournal ArticleResearch Support, Non-U.S. Gov't

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H Schneider, K Huse, G Birkenmeier, A Otto, G H Scholz. 1996-10-01. Gene transfer mediated by alpha2-macroglobulin.. https://doi.org/10.1093/nar%2F24.19.3873

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