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PubMed · 8890595

[Three-dimensional analysis of chromosomes by a computerized fluorescence microscope system].

T Haraguchi·Y Hiraoka
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Abstract

In order to study spatial arrangement and dynamics of chromosomes and nuclear structures, we have developed a computerized fluorescence microscope workstation. This microscope workstation is capable of rapidly acquiring three-dimensional, multiple-wavelength image data and allows accurate reconstructions of three-dimensional cellular structures stained with multiple fluorescent dyes. Using this microscope system, we have examined dynamics of chromosomes and microtubules in mammalian culture cells by combination of high-resolution analysis in fixed specimens and time-lapse analysis in living cells. A key aspect of such microscopic approaches is a capability of detecting transient events during dynamic biological processes.

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AnimalsChromosomesHumansImage Processing, Computer-AssistedMicroscopy, FluorescenceEnglish AbstractJournal ArticleReview

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T Haraguchi, Y Hiraoka. 1996. [Three-dimensional analysis of chromosomes by a computerized fluorescence microscope system].. https://pubmed.ncbi.nlm.nih.gov/8890595/

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