A reliable Golgi-Kopsch modification.

J N Riley

doi:10.1016/0361-9230(79)90067-4

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A reliable Golgi-Kopsch modification.

J N Riley

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Abstract

A reliable Golgi-Kopsch variant is described. Tissue is fixed with 1% paraformaldehyde, 1% glutaraldehyde in 0.1 M phosphate buffer, pH 7.2. Tissue slabs (2.5--4 thick) are immersed for 5--7 days in a solution containing 3.5 g potassium dichromate, 15 g sucrose, 1.65 ml formaldehyde in 100 ml distilled water, washed briefly in 0.75% silver nitrate, and immersed in 0.75% silver nitrate for 2--3 days. Procedures for cutting, counterstaining, and handling sections are described.

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Animals Female Hippocampus Histological Techniques Rats Staining and Labeling Journal Article Research Support, U.S. Gov't, P.H.S.

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J N Riley. A reliable Golgi-Kopsch modification.. https://doi.org/10.1016/0361-9230(79)90067-4

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