Quantitative PCR with colorimetric detection.

F Demay; S Darche; P Kourilsky; S Delassus

doi:10.1016/0923-2494(96)82049-9

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PubMed · 8903107

Quantitative PCR with colorimetric detection.

F Demay·S Darche·P Kourilsky·S Delassus

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Abstract

We describe a quantitative polymerase chain reaction (PCR) technique with a colorimetric read-out, which enables quantitation of various sequences on a single microplate within one day. Using the quantitation of human IL10 and beta-actin transcripts, we show that this technique provides better reproducibility, as results are derived from a series of PCRs made with various amounts of standard. This also enables the control of the consistency of the data and elimination of some artifactual results.

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Actins Colorimetry Humans Interleukin-10 Polymerase Chain Reaction Reproducibility of Results Sensitivity and Specificity Journal Article Research Support, Non-U.S. Gov't

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F Demay, S Darche, P Kourilsky, S Delassus. Quantitative PCR with colorimetric detection.. https://doi.org/10.1016/0923-2494(96)82049-9

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