[NMR and other physical methods].

H Akutsu

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PubMed · 8904229

[NMR and other physical methods].

H Akutsu

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Abstract

Solid-state NMR can be used for the samples under limited motions such as membrane systems with membrane transport proteins. Nuclear spin interactions such as the chemical shift anisotropy, dipole-dipole interaction and quadrupole interaction can provide the structural information. To get the information at high resolution, the sample should be either oriented or rotated at the magic angle. New methods are developed to recover the nuclear spin interactions under magic angle spinning. By using specifically deuterated phospholipids, detailed information on the protein-lipid interactions can be obtained from the quadrupole splittings of these phospholipids. X-ray crystallography has contributed to the structural analysis of membrane transport proteins after the crystallization method in the presence of detergents was developed.

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Animals Carrier Proteins Crystallography, X-Ray Magnetic Resonance Spectroscopy Membrane Lipids Phospholipids Protein Conformation English Abstract Journal Article Review

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H Akutsu. 1996. [NMR and other physical methods].. https://pubmed.ncbi.nlm.nih.gov/8904229/

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