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PubMed · 8908518

Real time quantitative PCR.

C A Heid·J Stevens·K J Livak·P M Williams
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Abstract

We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.

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Cell LineDNA, ComplementaryFactor VIIIHumansPolymerase Chain ReactionTransfectionJournal Article

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BibTeXRIS

C A Heid, J Stevens, K J Livak, P M Williams. 1996. Real time quantitative PCR.. https://doi.org/10.1101/gr.6.10.986

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