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PubMed · 890867

Interactions between fluorescent labeled phosphatidyl serine and cations.

W E Harris
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Abstract

Phosphatidyl serine (PS) was reacted with dimethylaminoanphthalenesulfonyl (DNS) chloride to produce a fluorescent phospholipid derivative (DNS--PS). By comparison of the emission maximum of the fluorochrome in solvents of varying dielectric constants and in aqueous suspension it was concluded that the DNS moiety of sonicated DNS--PS micells was within the glycerol region of the lipid. Addition of CaC12 to aqueous suspensions of DNS--PS micells caused a shift in the emission maximum from 524 nm to 490 nm, a two-fold increase in the relative fluorescence and an increase in the polarization of fluorescence and finally aggregation. MgC12 produced similar but smaller changes in the fluorescence parameters. In the presence of calcium the thermal transition of the fatty acid tail region could be monitored as an increase in the fluorescence intensity of the DNS group. Without calcium an almost linear decrease in relative fluorescence was observed. These data suggested that calcium caused the fluorochrome to be shifted from the glycerol region of the lipid structure into the fatty acid tail region. These observations indicate how the conformation of a biological membrane might be altered by the interaction between acidic phospholipids and calcium.

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Binding SitesCalciumDansyl CompoundsLightMembranes, ArtificialModels, BiologicalPhosphatidylserinesScattering, RadiationSolventsSpectrometry, FluorescenceTemperatureJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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BibTeXRIS

W E Harris. 1977. Interactions between fluorescent labeled phosphatidyl serine and cations.. https://doi.org/10.1016/0009-3084(77)90046-9

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