Bacillus caldolyticus prs gene encoding phosphoribosyl-diphosphate synthase.

B N Krath; B Hove-Jensen

doi:10.1016/0378-1119(96)00222-3

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Bacillus caldolyticus prs gene encoding phosphoribosyl-diphosphate synthase.

B N Krath·B Hove-Jensen

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Abstract

The prs gene, encoding phosphoribosyl-diphosphate (PRPP) synthase, as well as the flanking DNA sequences were cloned and sequenced from the Gram-positive thermophile, Bacillus caldolyticus. Comparison with the homologous sequences from the mesophile, Bacillus subtilis, revealed a gene (gcaD) encoding N-acetylglucosamine-1-phosphate uridyltransferase upstream of prs, and a gene homologous to ctc downstream of prs. cDNA synthesis with a B. caldolyticus gcaD-prs-ctc-specified mRNA as template, followed by amplification utilising the polymerase chain reaction indicated that the three genes are co-transcribed. Comparison of amino acid sequences revealed a high similarity among PRPP synthases across a wide phylogenetic range. An E. coli strain harbouring the B. caldolyticus prs gene in a multicopy plasmid produced PRPP synthase activity 33-fold over the activity of a haploid B. caldolyticus strain. B. caldolyticus PRPP synthase was resistant to heat treatment at 70 degrees C to a much higher extent than PRPP synthase from B. subtilis.

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Amino Acid Sequence Bacillus Bacterial Proteins Base Sequence Cloning, Molecular DNA, Bacterial Molecular Sequence Data Ribose-Phosphate Pyrophosphokinase Sequence Homology, Amino Acid Transcription, Genetic Transferases (Other Substituted Phosphate Groups)Journal Article Research Support, Non-U.S. Gov't

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B N Krath, B Hove-Jensen. 1996-10-17. Bacillus caldolyticus prs gene encoding phosphoribosyl-diphosphate synthase.. https://doi.org/10.1016/0378-1119(96)00222-3

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