Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally controlled, competitive differential PCR (CD-PCR).

S Nagel; M Schmidt; C Thiede; D Huhn; A Neubauer

doi:10.1093/nar/24.20.4102

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Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally controlled, competitive differential PCR (CD-PCR).

S Nagel·M Schmidt·C Thiede·D Huhn·A Neubauer

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Abstract

The quantification of Bcr-Abl transcript numbers in chronic myelogenous leukemia (CML) patients described here uses simultaneous competitive PCR amplification of the target gene (Bcr-Abl) and a reference gene (porphobilinogen deaminase; Pbgd) together with a single composite competitor molecule for both targets based on heterologous sequences. Using this technique, Bcr-Abl transcript numbers could be reproducibly determined even in clinical samples known to harbour poor quality RNA.

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DNA Primers DNA, Complementary Electrophoresis, Agar Gel Gene Expression Regulation, Neoplastic Genes, abl Genetic Vectors Humans Hydroxymethylbilane Synthase Interferons Leukemia, Myelogenous, Chronic, BCR-ABL Positive Polymerase Chain Reaction Transcription, Genetic Journal Article Research Support, Non-U.S. Gov't

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S Nagel, M Schmidt, C Thiede, D Huhn, A Neubauer. 1996-10-15. Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally controlled, competitive differential PCR (CD-PCR).. https://doi.org/10.1093/nar%2F24.20.4102

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