Neuronal nitric oxide synthase expression in neuronal cell differentiation.

T Ogura; K Nakayama; H Fujisawa; H Esumi

doi:10.1016/0304-3940(96)12324-7

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Neuronal nitric oxide synthase expression in neuronal cell differentiation.

T Ogura·K Nakayama·H Fujisawa·H Esumi

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Abstract

Expression of neuronal nitric oxide synthase (n-NOS) was investigated during neuronal cell differentiation. Trace amounts of n-NOS mRNA were detected in the primary culture of neural precursor cell (NPC) at embryonic day 10, and a high level of n-NOS mRNA was observed after a further 7 days cultivation of NPC. Both n-NOS activity and its mRNA level were also increased in the human neuroblastoma cell line, TGW, following trans-retinoic acid (RA)-induced neuronal differentiation. These findings indicate that there is positive regulation of n-NOS mRNA during neuronal cell differentiation. However TGW cell differentiation with trans-RA could not be reversed by treatment with specific inhibitors of NOS. Thus, the signal transduction mechanism which is involved in the induction of neuronal cell differentiation by trans-RA appears to be distinct from the NO-mediated pathway.

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Animals Base Sequence Cell Differentiation Cells, Cultured Mice Mice, Inbred BALB C Mice, Inbred DBA Molecular Sequence Data Neuroblastoma Neurons Nitric Oxide Synthase Rats Signal Transduction Tretinoin Tumor Cells, Cultured Journal Article Research Support, Non-U.S. Gov't

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T Ogura, K Nakayama, H Fujisawa, H Esumi. 1996-02-02. Neuronal nitric oxide synthase expression in neuronal cell differentiation.. https://doi.org/10.1016/0304-3940(96)12324-7

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