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flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis.

M H Rashid·J Sekiguchi
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Abstract

A flaD (sinR) null mutation depressed sigD-lacZ expression only two- to fourfold, whereas a flaD1 point mutation depressed it almost completely. Introduction of pHYSigD, a sigmaD-overproducing plasmid, corrected the filamentous phenotype common to both sinR mutants; autolysin synthesis was restored partially and completely in the flaD1 and flaD (sinR) null strains, respectively. Flagellin synthesis and motility were not restored at all in either strain.

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Bacillus subtilisBacterial ProteinsDNA-Directed RNA PolymerasesFlagellinGene Expression Regulation, BacterialMutationN-Acetylmuramoyl-L-alanine AmidaseSigma FactorJournal ArticleResearch Support, Non-U.S. Gov't

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M H Rashid, J Sekiguchi. 1996. flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis.. https://doi.org/10.1128/jb.178.22.6640-6643.1996

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