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Enzymic determinations with rotating bioreactors and continuous-flow-stopped-flow processing. Determination of choline esters in pharmaceuticals.

B López Ruiz·M Sánchez-Cabezudo·H A Mottola
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Abstract

The neuromuscular blocking agent, succinylcholine, and the neurotransmitter, acetylcholine, have been determined in pharmaceutical preparations utilizing a sensing unit comprised of a rotating bioreactor and an amperometric detector. The bioreactor consisted of a rotating disk made of Teflon (1 cm id) with cholinesterase (EC 3.1.1.8) and choline oxidase (EC 1.1.3.17) co-immobilized on the top surface of the disk. Amperometric detection of H2O2 and initial rate measurements were employed for quantification.

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AcetylcholineAlcohol OxidoreductasesBioreactorsCholinesterasesEnzymes, ImmobilizedPharmaceutical PreparationsSuccinylcholineJournal ArticleResearch Support, Non-U.S. Gov't

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B López Ruiz, M Sánchez-Cabezudo, H A Mottola. 1996. Enzymic determinations with rotating bioreactors and continuous-flow-stopped-flow processing. Determination of choline esters in pharmaceuticals.. https://doi.org/10.1039/an9962101695

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