Expression of human decay accelerating factor (hDAF) in transgenic pigs regulates complement activation during ex vivo liver perfusion--immunopathological findings.

A Pascher; C Poehlein; M Storck; D Abendroth; J Mueller-Hoecker; W Koenig; V K Young; D J White; C Hammer

doi:10.1007/978-3-662-00818-8_93

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Expression of human decay accelerating factor (hDAF) in transgenic pigs regulates complement activation during ex vivo liver perfusion--immunopathological findings.

A Pascher·C Poehlein·M Storck·D Abendroth·J Mueller-Hoecker·W Koenig·V K Young·D J White·C Hammer

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Abstract

Ex vivo perfusions of human decay accelerating factor-expressing transgenic (n = 3), and nontransgenic (n = 6) porcine livers with human blood revealed a higher degree of organ damage in non-transgenic pig livers. Transgenic livers were protected from immunohistologically detectable complement deposition, despite corresponding IgM and IgG deposits in both groups. Complement activation and consumption of C3 and C4 turned out to be lower in transgenic pig livers. In contrast to livers of normal landrace pigs, livers from genetically manipulated pigs showed no morphological alterations after perfusion.

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Animals Animals, Genetically Modified CD55 Antigens Complement Activation Humans Liver Perfusion Swine Journal Article

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A Pascher, C Poehlein, M Storck, D Abendroth, J Mueller-Hoecker, W Koenig, V K Young, D J White, C Hammer. 1996. Expression of human decay accelerating factor (hDAF) in transgenic pigs regulates complement activation during ex vivo liver perfusion--immunopathological findings.. https://doi.org/10.1007/978-3-662-00818-8_93

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