A method for introducing site-specific mutations using oligonucleotide primers and its application to site-saturation mutagenesis.

M J O'Donohue; G G Kneale

doi:10.1007/bf02740772

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A method for introducing site-specific mutations using oligonucleotide primers and its application to site-saturation mutagenesis.

M J O'Donohue·G G Kneale

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Abstract

Oligonucleotide primer-directed mutagenesis is a useful molecular biological tool, which is invaluable for the study of the structure/function relationships in proteins and for the creation of mutant proteins possessing modified or novel biological activities. Mutagenesis studies in which a site-saturation approach is employed require a high-efficiency mutagenesis procedure, which will generate a population of mutated molecules containing an even distribution of all possible amino acid changes, or a subset thereof. This article describes such a mutagenesis technique and discusses the adaptations that are necessary to perform site-saturation mutagenesis.

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DNA Primers Genetic Techniques Mutagenesis, Site-Directed Thionucleotides Journal Article

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M J O'Donohue, G G Kneale. 1996. A method for introducing site-specific mutations using oligonucleotide primers and its application to site-saturation mutagenesis.. https://doi.org/10.1007/bf02740772

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